赵祥吉,秦艳杰,李霞,刘洋,丁鉴峰

• 1:大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室
• 2:江西省上饶市鄱阳中学
• 3:大连海洋大学农业部北方海水增养殖重点实验室

摘要(Abstract)：

应用RACE法从刺参Apostichopus japonicus体腔细胞中克隆出26S蛋白酶体组成19S亚复合体亚基之一的S7亚基基因(GenBank登录号:JQ922514)。该基因的cDNA序列全长为2 445 bp,其中5'UTR为70bp,3'UTR为1 070 bp,开放阅读框为1 305 bp,编码435个氨基酸;保守区具有典型的ATP结合和水解基序,氨基酸序列分别为GPPGTGKT和DEID,具有MAT和VRPGRLDR的RNA/DNA螺旋酶的特征性基序;刺参26S蛋白酶体S7亚基基因与紫海胆的氨基酸序列同源性最高(95%),与脊椎动物、节肢动物的同源性较高(88%~89%),与线虫、拟南芥和酵母同源性较低(85%、77%和73%);S7亚基编码的蛋白没有信号肽,也没有跨膜结构域,为非跨膜蛋白,定位于细胞中的液态基质中。采用实时定量PCR方法检测了26S蛋白酶体S7亚基基因在刺参5种组织中的表达情况,结果显示,在肠、呼吸树、表皮、体腔细胞、纵肌中26S蛋白酶体S7基因均有明显表达,且体腔细胞中表达量最高,其次是纵肌和肠,在体壁和呼吸树中表达量较低。本研究中首次在刺参体内发现并克隆了26S蛋白酶体S7亚基基因,同时采用Realtime PCR技术对该基因的表达部位进行了研究,所得结果将为研究棘皮动物蛋白质代谢途径提供新的着眼点,为刺参生理功能的调控研究奠定了基础。

关键词(KeyWords)： 刺参;26S蛋白酶体S7亚基;基因克隆;组织表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30371099);; 辽宁省教育厅实验室专项(2008S064)

作者(Author): 赵祥吉,秦艳杰,李霞,刘洋,丁鉴峰

DOI: 10.16535/j.cnki.dlhyxb.2013.06.021

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