孙静娴;赵文;左中原;贾沁贤;

• 1:大连水产学院辽宁省省级高校水生生物学重点实验室,大连水产学院辽宁省省级高校水生生物学重点实验室,山西省水产研究所,中国地质科学研究院盐湖与热水资源研究中心 辽宁大连116023,辽宁大连116023,山西太原030006,北京100037

摘要：

采用热休克法抑制受精卵第一次卵裂诱导虹鳟Oncorhynchus mykiss四倍体,对影响热休克处理效果的3个参数:处理温度(26~31℃)、处理持续时间(3~16 min)、处理起始时刻(3 h 15 min~6 h 50min)进行了研究。结果表明:在授精后3 h 45 min,于31℃下热休克处理5、6、7 min,或30℃下处理7、10 min,或29℃下处理10 min,或28℃下处理16 min,都能成功地诱导出虹鳟四倍体胚胎,四倍体的诱导率为13%~33%,发眼期相对存活率为19.52%~96.54%。

关键词： 虹鳟;四倍体;热休克

Abstract: Tetraploids in rainbow trout Oncorhynchus mykiss were induced by suppressing the first cleavage by heat shock.The temperature(26-31 ℃),duration(3-16 min)and application time(3 h 15 min-6 h 50 min after egg activation)were investigated during the heat shock.The shock medium intensity including 31 ℃ for 5,6,and 7 min,30 ℃ for 7,and 10 min,29 ℃ for 10 min,and 28 ℃ for 16 min,and 3 h 45 min after fertilization were found to be the suitable treatment for induction of the tetraploidy rainbow trout with the tetraploid rate of 13%-33%,and the survival of 19.52% to 96.54%(relative to that of the diploid control)at the eyed stage.

Keywords: Oncorhynchus mykiss;heat shock;tetraploid

基金项目: 山西省科技厅科研攻关项目资助(012033)

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参考文献：

• [1]THORGAARD G H,JAZWIN M E,STIER A R.Polyploidy in-duced by heat shock in rainbow trout[J].Trans Am Fish Soc,1981,110:546-550.
• [2]CHOURROUTD.Tetraploidy induced by heat shocks in the rain-bow trout(Salmo gairdneriR.)[J].Reprod Nutr Dev,1982,22:569-574.
• [3]CHOURROUT D.Pressure-induced retention of second polarbody and suppression of first cleavage in rainbowtrout:productionof all-triploids,all-tetraploids,and heterozygous and homozy-gous diploid gynogenetics[J].Aquaculture,1984,36:111-126.
• [4]CHOURROUTD.Genetic manipulations in fish:review of methods[C]//Proc.World Symp.Select.Hybrid.Genet.Eng.Aquac-ult.Berlin:Hecncmann,1986:111-122.
• [5]DITER A,QUILLETE,CHOURROUTD.Suppression of first eggmitosis induced by heat shocks in the rainbow trout[J].Journalof Fish Biology,1993,42:777-786.
• [6]马涛,朱才宝,朱秉仁.热休克诱导虹鳟四倍体[J].水生生物学报,1987,11(4):329-334.
• [7]洪云汉.鱼类单个胚胎染色体标本的快速制备法[J].淡水渔业,1987(1):35-36.
• [8]CHOURROUT D,HAPPE A.Improved methods of direct chromo-some preparation in rainbow troutSalmo gairdneri[J].Aquacul-ture,1986,52:255-261.
• [9]BIDWELL C A,CHRISMAN C L,LIBEY G S.Polyploidy inducedby heat shock in channel catfish[J].Aquaculture,1985,51(1):25-32.
• [10]潘光碧,胡德高,邹桂伟,等.热休克诱导颖鲤四倍体的研究[J].水产学报,1997,21(增刊):1-8.
• [11]ONOZATO H.Diploidization of gynogenetically activated salmo-nid eggs using hydrostatic pressure[J].Aquaculture,1984,43:91-97.
• [12]CHOURROUT D.Thermal induction of diploid gynogenesis andtriploidy in the eggs of the rainbowtrout(Salmo gairdneriRichard-son)[J].Reprod Nutr Dev,1980,20:727-733.
• [13]LOUYD,PURDOMC E.Diploid gynogenesis induced by hydro-static pressure in rainbow trout,Salmo gairdneriRichardson[J].J Fish Biol,1984,24:665-670.
• [14]SOLAR I I,DONALDSONE M,HUNTER G A.Induction of trip-loidy in rainbow(Salmo gairdneriRichardson)by heat shock,andinvestigation of early growth[J].Aquaculture,1984,42:57-67.
• [15]LOU Y D,PURDOM C E.Polyploidy induced by hydrostaticpressure in rainbow trout,Salmo gairdneriRichardson[J].JFish Biol,1984,25,345-351.
• [16]SWARUP H.Effect of triploidy on the body size,general organi-zation and cellular structure inGasterosteus aculeatus(L.)[J].J Genet,1959,56:143-155.
• [17]PFEIFFER T,SCHRADER L E,BINGHAME T.Physiologicalcomparison of isogenic diploid-tetraploid,tetraploid-octoploidalfalfa populations[J].Crop Sci,1980,20:299-303.
• [18]LEARY R F,ALLENDORF F W,KNUDSEN K L.Develop-mental stability and enzyme heterozygosity in rainbow trout[J].Nature,1983,301:71-72.