刘青;

• 1:大连水产学院辽宁省省级高校水生生物学重点实验室 辽宁大连116023

摘要：

对日本高密度培养轮虫Brachionusspp.的方法、原理和技术进行了综述:1)采用浓缩的淡水小球藻作为新食物以取代微绿球藻和面包酵母;2)培养液中充入氧气以防止缺氧;3)自动添加HC l使pH控制在7左右,以促进总氨解离为NH4+和OH-;4)用尼龙滤板清除过量的悬浮有机质;5)加入去泡剂以减少培养液和轮虫被泡沫吸附而流失。采用这些技术可使轮虫培养密度增加到1~2万个/mL,比传统方法提高100倍以上,并大幅度降低了生产成本。最近,日本采用连续的滤膜装置(CMFU)开发了一种新的流水培养系统,其中NH4+和溶解有机质都被滤除,轮虫密度可达到16万个/mL,成功地开发了轮虫的超高密度培养。

关键词： 轮虫;高密度培养;日本

Abstract: This paper summarizes the progress on principle and technology of high-density rotifer in mass culture method in Japan.There are at least 100 times more efficient in Japanese mass rotifer culture method with less cost for production than that in the conventional method.Firstly,condensed freshwater Chlorella is employed as new food for rotifers instead of bakery yeast and Nannochloropsis.Secondly,cultures were generally supplied with constant O_2 to avoid oxygen deficiency.Thirdly,pH was adjusted to 7 by automatic addition of HCl to promote the dissociation of NH_3 to NH+_4 and OH-.Fourth,the excess suspended organic matter can be efficiently romoved by a nylon filtration mat.Fifth, addition of a defoamer substance can prevent the culture water from loss and from rotifer attachment to the foam.Using these techniques,the rotifer culture density increases to 20 000 ind./mL.Recently a novel culture system using a continuous membrane filtration unit(CMFU) for ultra-high-density culture is developed in Japan.In this culture system NH+_4 and soluble organic substances are removed by the culture liquid,with rotifer density of 160 000 ind./mL.

Keywords: rotifer;high density culture;Japan

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参考文献：

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