刘艳辉;李改娟;祖岫杰;张雅斌;陈伟强;郭军;赵全东;李刚;

• 1:吉林省水产科学研究院

摘要：

在实验室条件下,研究了不同水温(16、26℃)和不同药物剂量[15、45 mg/kg(鱼体质量)]下,氟苯尼考(FF)及其代谢物氟苯尼考胺(FFa)在德国镜鲤Cyprinus carpio mirror体内的残留消除规律。试验采用混饲口灌给药,在16、26℃水温条件下,以15 mg/kg(鱼体质量)剂量给药;在26℃水温条件下,以15、45 mg/kg(鱼体质量)剂量给药,于给药后0.5、1、2、4、6、8、12、24、72、120、168、216、264 h分别取鱼体肌肉、肝胰脏和肾脏组织,采用高效液相色谱荧光检测法测定鱼体各组织中FF和FFa的含量。结果表明:鱼体各组织中的FF和FFa,高温组较低温组消除快,高剂量组较低剂量组消除慢;高水温(26℃)下,用药后168 h时鱼体肌肉中的FF检测不出,而FFa浓度为36.3μg/kg,低水温(16℃)下,用药后264 h时鱼体肌肉中的FF浓度为23μg/kg,而FFa浓度为49μg/kg;低剂量组在用药后168 h时,鱼体肌肉中的FF和FFa的浓度之和为36μg/kg,而高剂量组在用药后264 h时,鱼体肌肉中的FF和FFa的浓度之和为59μg/kg;药物在德国镜鲤各组织中的消除顺序依次为肌肉>肝胰脏>肾脏。

关键词： 德国镜鲤;氟苯尼考;氟苯尼考胺;水温;剂量;残留

Abstract: The depletion of florfenicol and its metabolite florfenicol amine residues was studied in Germany mirror carp Cyprinus carpio mirror under different water temperature(16,26 ℃) and doses(15,45 mg/kg body weight)of the drug.The Germany mirror carp were orally administrated for the drug at a dose of 15 mg/kg body weight at water temperature of 26 ℃(group A) and 16 ℃(group B) only once,and at a dose of 15 mg/kg and 45 mg/kg body weight at water temperature of 26 ℃(group C).Then the muscle,hepatopancreas and kidney tissues were collected from the Germany mirror carp killed 0.5,1,2,4,6,8,12,24,72,120,168,216,and 264 h after oral drug administration.The florfenicol(FF) and florfenicol amine(FFa) levels in the tissues were detected by reverse phase high performance liquid chromatography(HPLC) with fluorescence detection.It was found that the FF and FFa in three tissues were more rapidly depleted and eliminated at high water temperature than at low water temperature,and slower at high dosage than at low dosage.At 26 ℃,the FFa level in the issues was found to be at 36.3 μg/kg and no FF was detected 168 h after oral drug administration.At 16 ℃,however,the FF level in the issues was at 23 μg/kg and the FFa at 49 μg/kg 264 h after oral drug administration.At a dosage of 15 mg/kg body weight,the sum of FF and FFa levels in the muscles was found to be 36 μg/kg 168 h after oral drug administration,and at a dosage of 45 mg/kg body weight,59 μg/kg 264 h after oral drug administration.The order of elimination of the drug in various tissues was ranged as: muscle >hepatopancreas >kidney.

Keywords: Cyprinus carpio mirror;florfenicol(FF);florfenicol amine(FFa);water temperature;dosage;residues

基金项目: 国家现代农业产业技术体系项目(CARS-46-29)

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