叶元土,林仕梅,罗莉,杨思华,陈文
摘要：
本试验测得南方大口鲶胃内、胆汁和肠内ｐＨ分别为５．５～６．５，６．５～７．０，６．５～７．５，长吻鱼危胃内、胆汁和肠内ｐＨ值分别为５．０～６．５，６．５～７．０，６．０～７．０。南方大口鲶胃、胰脏和肠蛋白酶的最适ｐＨ值分别为２．６、６．２和７．０，淀粉酶最适ｐＨ值分别为５．０、６．４和７．０；蛋白酶最适温度分别为３３、３９℃和４５℃，淀粉酶最适温度分别为４５、３９℃和４１℃。长吻鱼危胃、胰脏和肠蛋白酶最适ｐＨ值分别为３．０、６．２和７．４，淀粉酶最适ｐＨ值分别为６．６、６．６和７．４；蛋白酶最适温度为３９、３６℃和４１℃，淀粉酶最适温度分别为４５、３６℃和３９℃。两种鱼胃内ｐＨ值较高，均高于胃蛋白酶的最适ｐＨ值。而肠内ｐＨ值范围适合蛋白酶和淀粉酶最大活性的作用。两种鱼蛋白酶、淀粉酶的最适温度均高于鱼体生活的水环境温度。两种鱼肠蛋白酶活力受温度变化的影响较大。
关键词： pH值；温度；消化酶；南方大口鲶
Abstract: The pH values of the gastric juice,the bile and the intestinal juice are 55~65,65~70 and 65~75 in silurus meridionalis respectively and 56~65,65~70 and 60~70 in Leiocassis longirostrisThe optimum pH values of stomach, pancreas and intestine for the protease are 26,62 and 70 respectively, and for the amylase are 50,64 and 70 in Silurus meridionalisIn Leiocassis longirostris, the optimum pH values of stomach, pancreas and intestine for the protease are 30,62 and 74,and for the amylase are 66,66 and 74. The optimum temperatures of stomach, pancreas and intestine for protease are 33,39℃and 45℃,and for amylase are 45, 39℃and 41℃ in Silurus meridionalis The optium temperatures of stomach, pancreas and intestine for the protease are 39, 36℃ and 41℃,and for the amylase are 45, 36℃ and 39℃ in Leiocassis longirostris These results indicate that the pH values of the gastric juice are higher than the optium pH values of the stomach protease in the two fishesAnd the pH values of the intestinal juice are conductive to the intestine digstive enzymes in the two fishes
Keywords: pH value; temperature; digstive enzyme; Silurus meriolionalis and Leiocassis longirostris
基金项目: 四川省应用基础项目
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